Nebivolol hydrochloride in the presence of acidic medium reacts with excess amount of potassium bromide-bromate and remaining potassium bromide-bromate reacts with safranin to produce a pink colour. The final stock solution was made to produce 100 µg/ml with methanol. The λmax was found to be 534 nm for assay. The linearity was found in the concentration range of 10-50 µg/ml. The correlation coefficient was found 0.999. The regression equation was found as Y = 0.018x+0.008. The method has been validated according to ICH guidelines.
Loading....